Coding

Part:BBa_K2779900:Design

Designed by: Ethan Agena, Rochelin Dalangin, Anna Kim, Irene Shkolnikov   Group: iGEM18_UAlberta   (2018-10-10)


HemA from R. capsulatus


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1219
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Optimized for E. coli expression.


Source

Amplified using PCR from an operon synthesized as a gBlock from IDT containing HemA and a fluorescent marker. The original gene is from Rhodobacter capsulatus

References